Terms of Use
- Sanger Sequencing
- Sample Concentration and Volumn
|
DNA |
DNA Concentration (ng/µl) |
DNAVolumn (µl) |
|
PCR DNA（< 1000 bp） |
> 20 |
> 10 |
|
PCR DNA（> 1000 bp） |
> 40 |
> 10 |
|
Plasmid DNA |
> 200 |
> 10 |
- NGS Sequencing
- DNA
|
|
16S (Metagenomic) |
Third Generation Sequencing |
WES |
WGS |
Methylation |
DNA Flex |
TSO 500 |
QIA_panel |
|||
|
Nanodrop conc. (ng/μl) |
> 100 |
200~500 |
DNA > 100 |
FFPE > 200 |
> 200 |
> 200 |
> 100 |
DNA > 100 |
FFPE > 200 |
DNA > 100 |
FFPE > 200 |
|
Qubit conc. (ng/μl) |
DNA > 10 |
|
DNA > 10 |
FFPE > 20 |
10~200 |
DNA > 30 |
DNA > 10 |
DNA > 5 |
FFPE > 5 |
DNA > 5 |
FFPE > 20 |
|
Volumn (μl) |
> 20 |
> 20 |
> 20 |
> 20 |
> 20 |
> 20 |
> 30 |
> 20 |
> 30 |
20 |
|
- RNA
|
|
RNA QC |
mRNA |
FFPE RNA |
miRNA (microRNA) |
10X single cell |
BD single cell |
|
Nanodrop conc. (ng/μl) |
> 50 |
> 50 |
> 20 |
> 50 |
- |
- |
|
Qubit conc. (ng/μl) |
> 50 |
> 50 |
> 20 |
> 50 |
- |
- |
|
Volumn (μl) |
> 5 |
> 20 |
> 20 |
> 20 |
>100 |
>500 |
|
RIN |
- |
≥ 7 |
- |
≥ 8 |
- |
- |
|
DV 200 |
- |
- |
> 36.5 |
- |
- |
- |
|
Cell number (cell/μl) |
- |
- |
- |
- |
700-1200 |
>200 |
- Affymetrix
- Affymetrix Genome-Wide Human SNP Array 6.0
DNA Concentration > 50ng/µl, Volume 30µl。
OD 260/280 : 1.8-2.0, OD 260/230>1.0。 - AffymetrixGeneChip Human Genome U133 Plus 2.0
RNA Concentration > 600ng/µl, Volume 20µl。
RIN > 7。 - AffymetrixClariom D assays, Human
RNA Concentration > 170ng/µl, Volume 20µl。
RIN > 7。 - AffymetrixTranscriptiome Array | Gene Array (Human, Mouse, Rat)
RNA Concentration > 170ng/µl, Volume 20µl。
RIN > 7。 - Affymetrix Gene Chip miRNA 4.0 Array
RNA Concentration > 130ng/µl, Volume 20µl。
RIN > 7，OD260/280 > 1.95。 - Affymetrix Genome Wide CytoScan (750K, HD)
DNA Concentration > 50-100ng/µl, Volume 30µl。
OD 260/280 : 1.8-2.0, OD 260/230>1.0。 - Affymetrix Genome Wide OncoScan
DNA Concentration > 100ng/µl (NanoDrop), Volume 20µl。
DNA Concentration > 20ng/µl (Qubit), Volume 20µl。
- Agilent 2100 Bioanalyzer
RNA Concentration >50ng/µl，Volume 4µl。