Upright Confocal (LEICA TCS SP8X)
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Proposal(Please use the CGMH internet) |
| Publications | |
| Instrument Manager | |
| Name：Mr.chen | |
| Ms.Huang | |
| Mr.Chang | |
| Number：(03)3281200#7706 |
| 1. | Technical principle | ||||
| 2. | Facility Specifications | ||||
| I. | Type：Leica TCS SP8X | ||||
| II. | Light source： | ||||
| i. | Visible laser | ||||
| A. | White light laser system: pulsed laser | ||||
| B. | Super continuous exciting range: 470nm-670nm | ||||
| C. | 1nm adjustable | ||||
| D. | >1.5mW for each wavelength | ||||
| ii. | UV laser | ||||
| A. | 405nm laser | ||||
| B. | 50mW | ||||
| III. | Microscope：LEICA DM6000 CS Upright microscope 1 set | ||||
| IV. | Objective lens： | ||||
| i. | Plan APO 10x, dry, NA 0.40, WD≧2.2mm | ||||
| ii. | Plan APO 20x, NA 0.70, coverglass, WD≧0.59mm | ||||
| iii. | Plan APO 40x, oil, NA 1.30, WD≧0.24mm | ||||
| iv | Plan APO 63x, oil, NA 1.40, WD≧0.14mm | ||||
| v | Plan APO 100x, oil, NA 1.40, WD≧0.09mm | ||||
| vi | APO 10x UVI, water immersion, NA 0.30, LWD≧3.6mm | ||||
| vii | APO 20x UVI, water immersion, NA 0.50, LWD≧3.5mm | ||||
| viii | APO 40x UVI, water immersion, NA 0.80, LWD≧3.3mm | ||||
| V. | Scanner： | ||||
| i. | for UV/405 nm-VIS-IR-gSTED with 4 independent laser ports | ||||
| ii. | Light gate FLIM technology | ||||
| iii. | Dual scan Tandem scanner | ||||
| A. | High resolution scan mode: | ||||
| a. |
Scan field of view(FOV) at least 22 mm, speed 7fps @512x512 in FOV 22mm, 84fps @ 512 x 16 |
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| b. |
Max. scan resolution 8192 x 8192, 16 bits grey scale, digitalization 12bits or 18 bits. |
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| B. | High speed scan mode: | ||||
| a. | Resonent scanner 12kHz (bi-direction 24kHz) , speed 40fps @ 512x512, 420fps @ 512 x 16 | ||||
| b. |
24kHz max. scan resolution 832 x 832, 16 bits grey scale, digitalization 12bits or 18 bits |
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| VI. | CCD： | ||||
| i. | 1.4 Mpixel (1392 x 1040) | ||||
| ii. | Exposure time 4us-10min | ||||
| VII. | Image capture format： | ||||
| i. | Bright field | ||||
| ii. | Fluorescence | ||||
| iii. | Reflection | ||||
| iiii. | Normaski differential interference contrast (DIC) | ||||
| VIII. | Build-in Scanner' confocal detector： | ||||
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Multi-band Spectrophotometer（400-850 nm）with 4PMT tube (2 cooling hybrid GaAsP/APD detectors + 2 cooled PMT detectors) |
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| IX | Software： | ||||
| i. | Leica Application Suite X | ||||
| ii. | Imaris 3D/4D real time image software | ||||
| iii. | Metamorph image analysis software | ||||