長庚醫療財團法人- Cgmh

心血管疾病動物模式
幹細胞治療
基因轉殖動物模式

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(07) 731-7123 ext 8591

電子郵件信箱

leu@adm.cgmh.org.tw

Steve Leu obtained his Bachelor of Science degree from Department of Biology, National Sun Yat-Sen University, Taiwan in 1996. He went on to further his studies, working on characterization of mRNA splicing factors and generation of transgenic and knock-out mouse models, and received his PhD degree from Graduated Institute of Biomedical Sciences, Chang Gung University, Taiwan in 2005. He also received the PhD student thesis award of the Association of Anatomists of the Republic of China, scoring first place. During the postdoctoral training, he participated in generating conditional knockout mouse models to investigate the temporal and spatial-specific function of mRNA splicing factors in vivo, as well as in determining the relationship between mRNA splicing factors and apoptotic events. Besides, he also joined the cardiovascular research team in Kaohsiung Chang Gung Memorial Hospital, Taiwan, working on utilization of mesenchymal stem cell-based therapy in cardiovascular disease animal models. His research interests include disease animal models of cardiovascular disease, stem cell therapy, developmental programing of metabolic syndromes, and translational research in cardiovascular diseases.

Selected Recent Publications (Pubmed Search)

Chou CK, Chang YT, Korinek M, Chen YT, Yang YT, Leu S, Lin IL, Tang CJ, Chiu CC. The Regulations of Deubiquitinase USP15 and Its Pathophysiological Mechanisms in Diseases. International journal of molecular sciences, 18(3): (2017)

Tseng CC, Wang CC, Hsiao CC, Lu HI, Leu S, Chang HC, Huang KT, Fang WF, Chen YM, Liu SF, Yang CT, Lin MC, Yip HK. Time courses and value of circulating microparticles in patients with operable stage non-small cell lung cancer undergoing surgical intervention. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37(9): 11873-11882 (2016)

Chao YM, Tain YL, Leu S, Wu KL, Lee WC, Chan JY. Developmental programming of the metabolic syndrome: Next-generation sequencing analysis of transcriptome expression in a rat model of maternal high fructose intake. Sheng li xue bao : [Acta physiologica Sinica], 68(5): 557-567 (2016)

Tain YL, Lee WC, Wu KL, Leu S, Chan JY. Targeting arachidonic acid pathway to prevent programmed hypertension in maternal fructose-fed male adult rat offspring. The Journal of nutritional biochemistry, 38: 86-92 (2016)

Lee WC, Tain YL, Wu KL, Leu S, Chan JY. Maternal Fructose Exposure Programs Metabolic Syndrome-Associated Bladder Overactivity in Young Adult Offspring. Scientific reports, 6: 34669 (2016)

Leu S, Day YJ, Sun CK, Yip HK. tPA-MMP-9 Axis Plays a Pivotal Role in Mobilization of Endothelial Progenitor Cells from Bone Marrow to Circulation and Ischemic Region for Angiogenesis. Stem cells international, 2016: 5417565 (2016)

Hsu CN, Wu KL, Lee WC, Leu S, Chan JY, Tain YL. Aliskiren Administration during Early Postnatal Life Sex-Specifically Alleviates Hypertension Programmed by Maternal High Fructose Consumption. Frontiers in physiology, 7: 299 (2016)

Chai HT, Yip HK, Sun CK, Hsu SY, Leu S*. AG490 suppresses EPO-mediated activation of JAK2-STAT but enhances blood flow recovery in rats with critical limb ischemia. Journal of inflammation (London, England), 13: 18 (2016)

Shao PL, Liao JD, Wong TW, Wang YC, Leu S, Yip HK. Enhancement of Wound Healing by Non-Thermal N2/Ar Micro-Plasma Exposure in Mice with Fractional-CO2-Laser-Induced Wounds. PloS one, 11(6): e0156699 (2016)

Ko SF, Yip HK, Zhen YY, Lee CC, Li JH, Lee CC, Leu S, Huang CC, Ng SH, Lin JW. Cancer Patient-Derived Circulating Microparticles Enhance Lung Metastasis in a Rat Model: Dual-Source CT, Cellular, and Molecular Studies. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 18(4): 490-499 (2016)

Chen YL, Sun CK, Tsai TH, Chang LT, Leu S, Zhen YY, Sheu JJ, Chua S, Yeh KH, Lu HI, Chang HW, Lee FY, Yip HK. Adipose-derived mesenchymal stem cells embedded in platelet-rich fibrin scaffolds promote angiogenesis, preserve heart function, and reduce left ventricular remodeling in rat acute myocardial infarction. American journal of translational research, 7(5): 781-803 (2015)

Lee FY, Chen YL, Sung PH, Ma MC, Pei SN, Wu CJ, Yang CH, Fu M, Ko SF, Leu S, Yip HK. Intracoronary Transfusion of Circulation-Derived CD34+ Cells Improves Left Ventricular Function in Patients With End-Stage Diffuse Coronary Artery Disease Unsuitable for Coronary Intervention. Critical care medicine, 43(10): 2117-2132 (2015)

Sun CK†, Leu S†, Hsu SY, Zhen YY, Chang LT, Tsai CY, Chen YL, Chen YT, Tsai TH, Lee FY, Sheu JJ, Chang HW, Yip HK. Mixed serum-deprived and normal adipose-derived mesenchymal stem cells against acute lung ischemia-reperfusion injury in rats. American journal of translational research, 7(2): 209-231 (2015)

Sheu JJ, Lee FY, Wallace CG, Tsai TH, Leu S, Chen YL, Chai HT, Lu HI, Sun CK, Yip HK. Administered circulating microparticles derived from lung cancer patients markedly improved angiogenesis, blood flow and ischemic recovery in rat critical limb ischemia. Journal of translational medicine, 13: 59 (2015)

Tsai CY, Su CH, Leu S, Chang AY, Chan JY, Chan SH. Endogenous vascular endothelial growth factor produces tonic facilitation of cardiac vagal baroreflex via fetal liver kinase-1 in medulla oblongata. International journal of cardiology, 187: 421-425 (2015)

Tain YL, Wu KL, Lee WC, Leu S, Chan JY. Maternal fructose-intake-induced renal programming in adult male offspring. The Journal of nutritional biochemistry, 26(6): 642-650 (2015)

Tsai TH, Sun CK, Su CH, Sung PH, Chua S, Zhen YY, Leu S, Chang HW, Yang JL, Yip HK. Sitagliptin attenuated brain damage and cognitive impairment in mice with chronic cerebral hypo-perfusion through suppressing oxidative stress and inflammatory reaction. Journal of hypertension, 33(5): 1001-1013 (2015)

Chang MW, Chen CH, Chen YC, Wu YC, Zhen YY, Leu S, Tsai TH, Ko SF, Sung PH, Yang CC, Chiang HJ, Chang HW, Chen YT, Yip HK. Sitagliptin protects rat kidneys from acute ischemia-reperfusion injury via upregulation of GLP-1 and GLP-1 receptors. Acta pharmacologica Sinica, 36(1): 119-130 (2015)

Chua S, Lee FY, Tsai TH, Sheu JJ, Leu S, Sun CK, Chen YL, Chang HW, Chai HT, Liu CF, Lu HI, Yip HK. Inhibition of dipeptidyl peptidase-IV enzyme activity protects against myocardial ischemia-reperfusion injury in rats. Journal of translational medicine, 12: 357 (2014)

Sun CK, Lee FY, Kao YH, Chiang HJ, Sung PH, Tsai TH, Lin YC, Leu S, Wu YC, Lu HI, Chen YL, Chung SY, Su HL, Yip HK. Systemic combined melatonin-mitochondria treatment improves acute respiratory distress syndrome in the rat. Journal of pineal research, 58(2): 137-150 (2015)

Tsai CY, Chua S, Leu S, Chang AY, Chan JY, Chan SH. VEGF tonically sustains myocardial performance via fetal liver kinase-1 in the heart. International journal of cardiology, 177(2): 727-730 (2014)

Chen HH, Chang CL, Lin KC, Sung PH, Chai HT, Zhen YY, Chen YC, Wu YC, Leu S, Tsai TH, Chen CH, Chang HW, Yip HK. Melatonin augments apoptotic adipose-derived mesenchymal stem cell treatment against sepsis-induced acute lung injury. American journal of translational research, 6(5): 439-458 (2014)

Chen YT, Chiang HJ, Chen CH, Sung PH, Lee FY, Tsai TH, Chang CL, Chen HH, Sun CK, Leu S, Chang HW, Yang CC, Yip HK. Melatonin treatment further improves adipose-derived mesenchymal stem cell therapy for acute interstitial cystitis in rat. Journal of pineal research, 57(3): 248-261 (2014)

Ko SF, Yip HK, Leu S, Lee CC, Sheu JJ, Lee CC, Ng SH, Huang CC, Chen MC, Sun CK. Therapeutic potential of tacrolimus on acute myocardial infarction in minipigs: analysis with serial cardiac magnetic resonance and changes at histological and protein levels. BioMed research international, 2014: 524078 (2014)

Wang CC, Tseng CC, Hsiao CC, Chang HC, Chang LT, Fang WF, Leu S, Wang YH, Tsai TH, Yang CT, Chen CH, Yip HK, Ho CK, Lin MC. Circulating endothelial-derived activated microparticle: a useful biomarker for predicting one-year mortality in patients with advanced non-small cell lung cancer. BioMed research international, 2014: 173401 (2014)

Fan CQ†, Leu S†, Sheu JJ, Zhen YY, Tsai TH, Chen YL, Chung SY, Chai HT, Sun CK, Yang JL, Chang HW, Ko SF, Yip HK. Prompt bone marrow-derived mesenchymal stem cell therapy enables early porcine heart function recovery from acute myocardial infarction. International heart journal, 55(4): 362-371 (2014)

Tain YL, Leu S, Wu KL, Lee WC, Chan JY. Melatonin prevents maternal fructose intake-induced programmed hypertension in the offspring: roles of nitric oxide and arachidonic acid metabolites. Journal of pineal research, 57(1): 80-89 (2014)

Chen HH, Lin KC, Wallace CG, Chen YT, Yang CC, Leu S, Chen YC, Sun CK, Tsai TH, Chen YL, Chung SY, Chang CL, Yip HK. Additional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury. Journal of pineal research, 57(1): 16-32 (2014)

Sheu JJ, Lin PY, Sung PH, Chen YC, Leu S, Chen YL, Tsai TH, Chai HT, Chua S, Chang HW, Chung SY, Chen CH, Ko SF, Yip HK. Levels and values of lipoprotein-associated phospholipase A2, galectin-3, RhoA/ROCK, and endothelial progenitor cells in critical limb ischemia: pharmaco-therapeutic role of cilostazol and clopidogrel combination therapy. Journal of translational medicine, 12: 101 (2014)

Sun CK, Zhen YY, Leu S, Tsai TH, Chang LT, Sheu JJ, Chen YL, Chua S, Chai HT, Lu HI, Chang HW, Lee FY, Yip HK. Direct implantation versus platelet-rich fibrin-embedded adipose-derived mesenchymal stem cells in treating rat acute myocardial infarction. International journal of cardiology, 173(3): 410-423 (2014)

Sung PH, Chang CL, Tsai TH, Chang LT, Leu S, Chen YL, Yang CC, Chua S, Yeh KH, Chai HT, Chang HW, Chen HH, Yip HK. Apoptotic adipose-derived mesenchymal stem cell therapy protects against lung and kidney injury in sepsis syndrome caused by cecal ligation puncture in rats. Stem cell research & therapy, 4(6): 155 (2013)

Leu S, Lu HI, Sun CK, Sheu JJ, Chen YL, Tsai TH, Yeh KH, Chai HT, Chua S, Tsai CY, Chang HW, Lee FY, Yip HK. Retention of endothelial progenitor cells in bone marrow in a murine model of endogenous tissue plasminogen activator (tPA) deficiency in response to critical limb ischemia. International journal of cardiology, 170(3): 394-405 (2014)

Tseng CC, Wang CC, Chang HC, Tsai TH, Chang LT, Huang KT, Leu S, Yen CH, Liu SF, Chen CH, Yang CT, Yip HK, Lin MC. Levels of circulating microparticles in lung cancer patients and possible prognostic value. Disease markers, 35(5): 301-310 (2013)

Sheu JJ, Sung PH, Leu S, Chai HT, Zhen YY, Chen YC, Chua S, Chen YL, Tsai TH, Lee FY, Chang HW, Ko SF, Yip HK. Innate immune response after acute myocardial infarction and pharmacomodulatory action of tacrolimus in reducing infarct size and preserving myocardial integrity. Journal of biomedical science, 20: 82 (2013)

Chen YT, Tsai TH, Yang CC, Sun CK, Chang LT, Chen HH, Chang CL, Sung PH, Zhen YY, Leu S, Chang HW, Chen YL, Yip HK. Exendin-4 and sitagliptin protect kidney from ischemia-reperfusion injury through suppressing oxidative stress and inflammatory reaction. Journal of translational medicine, 11: 270 (2013)

Lee FY, Lu HI, Zhen YY, Leu S, Chen YL, Tsai TH, Chung SY, Chua S, Sheu JJ, Hsu SY, Chang HW, Sun CK, Yip HK. Benefit of combined therapy with nicorandil and colchicine in preventing monocrotaline-induced rat pulmonary arterial hypertension. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 50(3-4): 372-384 (2013)

Chua S, Sheu JJ, Chen YL, Chang LT, Sun CK, Leu S, Sung HC, Tsai TH, Chung SY, Yeh KH, Cho CL, Kao YH, Yip HK. Sitagliptin therapy enhances the number of circulating angiogenic cells and angiogenesis-evaluations in vitro and in the rat critical limb ischemia model. Cytotherapy, 15(9): 1148-1163 (2013)

Chen YT, Yang CC, Zhen YY, Wallace CG, Yang JL, Sun CK, Tsai TH, Sheu JJ, Chua S, Chang CL, Cho CL, Leu S, Yip HK. Cyclosporine-assisted adipose-derived mesenchymal stem cell therapy to mitigate acute kidney ischemia-reperfusion injury. Stem cell research & therapy, 4(3): 62 (2013)

Lin YC, Chiang CH, Chang LT, Sun CK, Leu S, Shao PL, Hsieh MC, Tsai TH, Chua S, Chung SY, Kao YH, Yip HK. Simvastatin attenuates the additive effects of TNF-alpha and IL-18 on the connexin 43 up-regulation and over-proliferation of cultured aortic smooth muscle cells. Cytokine, 62(3): 341-351 (2013)

Sun CK†, Leu S†, Sheu JJ, Tsai TH, Sung HC, Chen YL, Chung SY, Ko SF, Chang HW, Yip HK. Paradoxical impairment of angiogenesis, endothelial function and circulating number of endothelial progenitor cells in DPP4-deficient rat after critical limb ischemia. Stem cell research & therapy, 4(2): 31 (2013)

Lin YJ, Chung SY, Liang CD, Kuo HC, Huang CF, Chien SJ, Lin IC, Leu S, Sun CK, Ko SF, Sheu JJ, Yip HK. Impact of extracorporeal membrane oxygenation support on clinical outcome of pediatric patients with acute cardiopulmonary failure: a single-center experience. Biomedical journal, 36(1): 28-34 (2013)

Yen CH, Tsai TH, Leu S, Chen YL, Chang LT, Chai HT, Chung SY, Chua S, Tsai CY, Chang HW, Ko SF, Sun CK, Yip HK. Sildenafil improves long-term effect of endothelial progenitor cell-based treatment for monocrotaline-induced rat pulmonary arterial hypertension. Cytotherapy, 15(2): 209-223 (2013)

Chen CH, Chuang HC, Huang CC, Fang FM, Huang HY, Tsai HT, Su LJ, Shiu LY, Leu S, Chien CY. Overexpression of Rap-1A indicates a poor prognosis for oral cavity squamous cell carcinoma and promotes tumor cell invasion via Aurora-A modulation. The American journal of pathology, 182(2): 516-528 (2013)

Chang CL, Leu S, Sung HC, Zhen YY, Cho CL, Chen A, Tsai TH, Chung SY, Chai HT, Sun CK, Yen CH, Yip HK. Impact of apoptotic adipose-derived mesenchymal stem cells on attenuating organ damage and reducing mortality in rat sepsis syndrome induced by cecal puncture and ligation. Journal of translational medicine, 10: 244 (2012)

Yip HK, Chang YC, Wallace CG, Chang LT, Tsai TH, Chen YL, Chang HW, Leu S, Zhen YY, Tsai CY, Yeh KH, Sun CK, Yen CH. Melatonin treatment improves adipose-derived mesenchymal stem cell therapy for acute lung ischemia-reperfusion injury. Journal of pineal research, 54(2): 207-221 (2013)

Yip HK, Sun CK, Tsai TH, Sheu JJ, Kao YH, Lin YC, Shiue YL, Chen YL, Chai HT, Chua S, Ko SF, Leu S*. Tissue plasminogen activator enhances mobilization of endothelial progenitor cells and angiogenesis in murine limb ischemia. International journal of cardiology, 168(1): 226-236 (2013)

Yang CH, Sheu JJ, Tsai TH, Chua S, Chang LT, Chang HW, Lee FY, Chen YL, Chung SY, Sun CK, Leu S, Yen CH, Yip HK. Effect of tacrolimus on myocardial infarction is associated with inflammation, ROS, MAP kinase and Akt pathways in mini-pigs. Journal of atherosclerosis and thrombosis, 20(1): 9-22 (2013)

Sheu JJ, Lin KC, Tsai CY, Tsai TH, Leu S, Yen CH, Chen YL, Chang HW, Sun CK, Chua S, Yang JL, Yip HK. Combination of cilostazol and clopidogrel attenuates rat critical limb ischemia. Journal of translational medicine, 10: 164 (2012)

Chen YT, Cheng BC, Ko SF, Chen CH, Tsai TH, Leu S, Chang HW, Chung SY, Chua S, Yeh KH, Chen YL, Yip HK. Value and level of circulating endothelial progenitor cells, angiogenesis factors and mononuclear cell apoptosis in patients with chronic kidney disease. Clinical and experimental nephrology, 17(1): 83-91 (2013)

Yen CH, Sun CK, Leu S, Wallace CG, Lin YC, Chang LT, Chen YL, Tsa TH, Kao YH, Shao PL, Hsieh CY, Chen YT, Yip HK. Continuing exposure to low-dose nonylphenol aggravates adenine-induced chronic renal dysfunction and role of rosuvastatin therapy. Journal of translational medicine, 10: 147 (2012)

Chen CH, Chang LT, Tung WC, Chen YL, Chang CL, Leu S, Sun CK, Tsai TH, Tsai IT, Chang HW, Yip HK. Levels and values of circulating endothelial progenitor cells, soluble angiogenic factors, and mononuclear cell apoptosis in liver cirrhosis patients. Journal of biomedical science, 19: 66 (2012)

Wang HT, Liu CF, Tsai TH, Chen YL, Chang HW, Tsai CY, Leu S, Zhen YY, Chai HT, Chung SY, Chua S, Yen CH, Yip HK. Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice. Journal of translational medicine, 10: 145 (2012)

Tsai TH, Chai HT, Sun CK, Yen CH, Leu S, Chen YL, Chung SY, Ko SF, Chang HW, Wu CJ, Yip HK. Obesity suppresses circulating level and function of endothelial progenitor cells and heart function. Journal of translational medicine, 10: 137 (2012)

Chua S†, Leu† S, Sheu JJ, Lin YC, Chang LT, Kao YH, Yen CH, Tsai TH, Chen YL, Chang HW, Sun CK, Yip HK. Intra-coronary administration of tacrolimus markedly attenuates infarct size and preserves heart function in porcine myocardial infarction. Journal of inflammation (London, England), 9(1): 21 (2012)

Chen YL, Chang CL, Sun CK, Wu CJ, Tsai TH, Chung SY, Chua S, Yeh KH, Leu S, Sheu JJ, Lee FY, Yen CH, Yip HK. Impact of obesity control on circulating level of endothelial progenitor cells and angiogenesis in response to ischemic stimulation. Journal of translational medicine, 10: 86 (2012)

Yen CH, Lin KC, Leu S, Sun CK, Chang LT, Chai HT, Chung SY, Chang HW, Ko SF, Chen YT, Yip HK. Chronic exposure to environmental contaminant nonylphenol exacerbates adenine-induced chronic renal insufficiency: role of signaling pathways and therapeutic impact of rosuvastatin. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 46(5): 455-467 (2012)

Leu S, Lin YM, Wu CH, Ouyang P. Loss of Pnn expression results in mouse early embryonic lethality and cellular apoptosis through SRSF1-mediated alternative expression of Bcl-xS and ICAD. Journal of cell science, 125(Pt 13): 3164-3172 (2012)

Tsai TH, Chen YL, Lin HS, Liu CF, Chang HW, Lu CH, Chang WN, Chen SF, Wu CJ, Leu S, Ko SF, Yip HK. Link between lipoprotein-associated phospholipase A2 gene expression of peripheral-blood mononuclear cells and prognostic outcome after acute ischemic stroke. Journal of atherosclerosis and thrombosis, 19(6): 523-531 (2012)

Chung SY, Sheu JJ, Lin YJ, Sun CK, Chang LT, Chen YL, Tsai TH, Chen CJ, Yang CH, Hang CL, Leu S, Wu CJ, Lee FY, Yip HK. Outcome of patients with profound cardiogenic shock after cardiopulmonary resuscitation and prompt extracorporeal membrane oxygenation support. A single-center observational study. Circulation journal : official journal of the Japanese Circulation Society, 76(6): 1385-1392 (2012)

Sun CK, Chang CL, Lin YC, Kao YH, Chang LT, Yen CH, Shao PL, Chen CH, Leu S*, Yip HK. Systemic administration of autologous adipose-derived mesenchymal stem cells alleviates hepatic ischemia-reperfusion injury in rats. Critical care medicine, 40(4): 1279-1290 (2012)

Yeh KH, Tsai TH, Chai HT, Leu S, Chung SY, Chua S, Chen YL, Lin HS, Yuen CM, Yip HK. Comparison of acute versus convalescent stage high-sensitivity C-Reactive protein level in predicting clinical outcome after acute ischemic stroke and impact of erythropoietin. Journal of translational medicine, 10: 6 (2012)

Yeh KH, Sheu JJ, Lin YC, Sun CK, Chang LT, Kao YH, Yen CH, Shao PL, Tsai TH, Chen YL, Chua S, Leu S*, Yip HK. Benefit of combined extracorporeal shock wave and bone marrow-derived endothelial progenitor cells in protection against critical limb ischemia in rats. Critical care medicine, 40(1): 169-177 (2012)

陳俐璇

邱于均

林佑宏

林宥禎

蔡秀玟

Education

1998-2005 Ph.D. Graduate Institute of Basic Medical Science,
Chang Gung University, Taoyuan, Taiwan

1996-1998 M.S. Graduate Institute of Basic Medical Science,
Chang Gung University, Taoyuan, Taiwan

1992-1996 B.S. Department of Biology, National Sun-Yet-Sen University,
Kaohsiung, Taiwan

Experience

2011- Assistant Professor, Kaohsiung Chang Gung Memorial Hospital
2009-2011 Adjunct Assistant Professor, Meiho University
2005-2011 Postdoctal fellow, Chang Gung University

Research Interests and experiments

Cardiovascular disease
Animal model of disease
Cytotherapy
Transgenic animal model
Transcriptional regulation and mRNA splicing
Cell differentiation and Apoptosis

Awards

The AAROC 9^th Ph.D. student thesis award, excellent work, 1^st place

Publications: (Pubmed Search)