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正立白光雷射掃描共軛焦顯微鏡 (LEICA TCS SP8X)

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	研究發表
	設備聯絡人
	姓名：陳志俊
	黃美綾
	章譽
	電話：(03)3281200
	分機：7706

1	技術原理
2	儀器規格
	I.	機型：Leica TCS SP8X
	II.	光源：
		i.	Visible laser
			A.	White light laser system: pulsed laser
			B.	Super continuous exciting range: 470nm-670nm
			C.	1nm adjustable
			D.	>1.5mW for each wavelength
		ii.	UV laser
			A.	405nm laser
			B.	50mW
	III.	顯微鏡：LEICA DM6000 CS 正立顯微鏡壹台
	IV.	物鏡：
		i.	Plan APO 10x, dry, NA 0.40, WD≧2.2mm
		ii.	Plan APO 20x, NA 0.70, coverglass, WD≧0.59mm
		iii.	Plan APO 40x, oil, NA 1.30, WD≧0.24mm
		iv.	Plan APO 63x, oil, NA 1.40, WD≧0.14mm
		v.	Plan APO 100x, oil, NA 1.40, WD≧0.09mm
		vi.	APO 10x UVI, water immersion, NA 0.30, LWD≧3.6mm
		vii.	APO 20x UVI, water immersion, NA 0.50, LWD≧3.5mm
		viii.	APO 40x UVI, water immersion, NA 0.80, LWD≧3.3mm
	V.	掃瞄器：
		i.	for UV/405 nm-VIS-IR-gSTED with 4 independent laser ports
		ii.	Light gate FLIM technology
		iii.	Dual scan Tandem scanner
			A.	High resolution scan mode:
				a.	Scan field of view(FOV) at least 22 mm, speed 7fps@512x512 in FOV 22mm, 84fps @ 512 x 16
				b.	Max. scan resolution 8192 x 8192, 16 bits grey scale, digitalization 12bits or 18 bits.
			B.	High speed scan mode:
				a.	Resonent scanner 12kHz (bi-direction 24kHz) , speed 40fps @ 512x512, 420fps @ 512 x 16
				b.	24kHz max. scan resolution 832 x 832, 16 bits grey scale, digitalization 12bits or 18 bits
	VI.	CCD：
		i.	1.4 Mpixel (1392 x 1040)
		ii.	Exposure time 4us-10min
	VII.	影像擷取形式：
		i.	Bright field
		ii.	Fluorescence
		iii.	Reflection
		iiii.	Normaski differential interference contrast (DIC)
	VIII.	Build-in Scanner' confocal detector：
		Multi-band Spectrophotometer（380-800 nm）with 4PMT tube (2 cooling hybrid GaAsP/APD detectors + 2 cooled PMT detectors)
	IX.	軟體：
		i.	Leica Application Suite X
		ii.	Imaris 3D/4D real time image software
		iii.	Metamorph image analysis software